Product documentation These safe alternatives are gentle toward tissue and do not cause shrinkage or morphology changes. Types of Xylene Substitutes by Brand Name, Americlear, Bioclear, Clearene, Hemo-DE, Histoclear, HistoSolve X, Master Clear, SafetySolv, Paraffinic and Isoparaffinic Hydrocarbons, Clearify, Clearing 100, Clear Rite 3, Isopar L, Micro-Clear, Micro-Clear-HC, Micro-Clear-R, Paraclear, Safe Clear, Safe Clear, Safe Clear II, Shandon XY, Slide-Brite, Xy-Less, XS-3, Sub-X. The majority of cell and tissue components have no natural color and are not visible. Minimal tissue shrinkage; Dries slowly with no … A xylene substitute for use in histology and cytology. …embedding system and is performed directly after the histoprocessing. Neo-Clear. For this method to be successful higher wax temperatures are required so that isopropanol can be eliminated from specimens during infiltration. Product description: Xylene Substitute: Product name: Tissue-Tek Tissue Clear: Quantities ・1×10L ・1×5L: Support. Even though the negative health effects of xylene have been known for more than 60 years, some labs continue to use this solvent when alternatives exist. This research led to the development of naphthenic solvents that are similar in performance. Tissue-Tek Tissue-Clear is compatible with Tissue-Tek ® Glas™ Tissue-Mount™ for glass coverslipping. Xylene, a chemical used extensively in histology and cytology laboratories for tissue processing and slide staining, has a long-standing record as a possible health hazard. Today, histology, pathology, cytology, research, veterinary, parasitology and dermatopathology labs should work to provide a safe environment for personnel by helping them avoid the toxic and dangerous effects of xylene. Histo-Clear enhances the clarity and vibrance of acidophilic stains and improves staining of Harris' Hematoxylin with a brighter Eosin background. Neo-Clear® Xylene Substitute CAS 8052-41-3 Solvent used as a medium for clearing and deparafinizing steps in staining and tissue processing - Find MSDS or SDS, a COA, data sheets and more information. Neo-Clear. Neo-Clear Xylene Substitute. 17. There are many safer options available. Tissue Clearing Agents Thermo Scientific™ Richard-Allan Scientific™ Signature Series Clear-Rite™ 3 Render complete deparaffinization and clearing during the staining process with Thermo Scientific™ Richard-Allan Scientific™ Signature Series Clear-Rite™ 3. Xylene Substitute. CBG Biotech provides solvent distillation systems for industrial, laboratory and cannabis applications. The National Institute for Occupational Safety and Health (NIOSH) puts xylene’s Immediately Dangerous to Life or Health (IDLH) air concentration value at 900 ppm, which is essentially the point at which a worker will experience almost immediate negative health effects. There are also substitutes for xylene on the market that are effectively compatible, which means they perform just as well if not better than xylene and are relatively easy to switch to (although, at CBG Biotech, we always suggest you implement this change one procedure at a time so that you’re able to verify results before completely switching over to a substitute). Rapid Clearing without the Toxic Hazards of Xylene In terms of performance, the d-limonenes are the next best type of xylene substitute. Xylene Substitute can be recycled with solvent recyclers. Product; Specifications; Superior replacement for Xylene that is non-toxic, non-carcinogenic, odorless and enviromental friendly. Hydrate the Section. D-limonene solvents also dry very slowly compared to xylene and they often leave an oily residue. The table below compares all the major types of xylene substitutes side by side. The first step in performing an H&E stain is to dissolve all the wax away with xylene (a hydrocarbon solvent). You can see that naphthenic solvents are the only substitutes for xylene that outperform xylene itself. Neo-Clear can be used in tissue preparations to remove alcohol and other dehydrating agents from the tissue before embedding. Histo-Clear is excellent for preserving fine tissue structure, and can often be used in place of xylene with no alteration of protocol. Tissue-Tek® Tissue Clear® Xylene Substitute. It is less oily than other solvent substitutes and therefore dries quickly and does not leave a residue.. Clearene penetrates tissue rapidly without overhardening and is safe to use with all open and closed system tissue processors. Xylene Substitute is a flammable product and must be stored in a flammable fire cabinet. Histo-Clear can directly substitute for xylene and yields excellent results in automated tissue processing. Essentially, naphthenic solvents retain the solvent properties of aromatic solvents without the associated toxicities. Hydrate the Section. It is intended to be used as a replacement for xylene where the hazards associated with aromatic hydrocarbon vapors are to be reduced. Histo-Clear leaves tissue less hard and brittle than xylene, facilitating the cutting of thin sections and prolonging microtome blade life. Research conducted by CBG Founder and Chairman, Gerald W. Camiener, Ph.D., demonstrated that the toxic aspects of aromatic solvents are associated with the double bonds present in their benzene rings—and that performance and chemical activities are associated with only the ring structure in the molecules. Naphthenic solvents are clearly the best choices for replacing xylene and other aromatic solvents. However, they vary greatly in efficacy. There are several types of xylene substitutes in the market today including both standard hydrocarbon and citrus limonene types that contain overpowering odors. Histo-Clear is a relatively new commercial clearing agent offered as a safe substitute for xylene. Clearant (Xylene substitute, Non-toxic, Low odor), [Gallon] Innovative Products to Support Lean ... Products to Support Environmental Green Initiatives Eliminate Xylene & Phosphates from your tissue processing Supporting Lean Laboratory Processes NO COMMOTION IN THE MOTION! Image-iT Plant Tissue Clearing Reagent and Image-iT Plant Tissue Hard-Set Mountant are based on Visikol™ for Plant Biology™ and Visikol™ Mount. Electron Microscopy Sciences Histosol™ Histosol with Xylene Substitute 1 GL. Tissue Clearing Agents Thermo Scientific™ Richard-Allan Scientific™ Xylenes (ACS) Ensure high-quality and consistent results with reagent grade Thermo Scientific™ Richard-Allan Scientific™ Xylenes (ACS). Although xylene is used widely as a clearing agent for tissue processing it is a toxic reagent and some laboratories prefer to use less-toxic alternatives such as isopropanol or other xylene substitutes. XYLENE SUBSTITUTES FOR HISTOLOGY DESCRIPTION: Rowley Biochemical offers two non-toxic, effective xylene substitutes for dewaxing and clearing in histologic applications. Eliminate Xylene & Phosphates from your tissue processing Supporting Lean Laboratory Processes NO COMMOTION IN THE MOTION! The first step in performing an H&E stain is to dissolve all the wax away with xylene (a hydrocarbon solvent). 3 In histopathology, xylene is routinely used clearing agent in tissue processing. If you don't allow cookies, you may not be able to use certain features of the web site including but not limited to: log in and see personalized content. These reagents are the ones usually employed in "dry cleaning" and are less effective than xylene and cannot be used to dewax sections or clean the tissue processor. For this method to be successful higher wax temperatures are required so that isopropanol can be eliminated from specimens during infiltration. SAFE-CLEAR? Everclear™ Xylene Substitute. SAFE-CLEAR? The web site is now storing only essential cookies on your computer. hbspt.cta._relativeUrls=true;hbspt.cta.load(2817229, 'c238788a-582c-4e65-937a-4262e3ac8a05', {}); David Camiener operated AMRESCO, Inc. a biochemical manufacturer and founded Pragmatic Materials, Inc., a supplier of regulated materials and high purity biochemicals to the Pharmaceutical and Life Science industries. Nuclear morphology is rendered in fine detail. The poorest-performing substitutes for xylene are the paraffinic and isoparaffinic hydrocarbons and the mixtures of paraffinic hydrocarbons with glycol ethers. Chemically, these newer, safer solvents are naphthenic hydrocarbons. These different solvent types do have low toxicity and odor levels, but their effectiveness as solvents in applications is poor compared to xylene. Xylene Substitute. Hematology labs that test blood rely on both of these chemicals as solvents, while hospital laboratories can use either chemical in preparing tissue … Histo-Clear can directly substitute for xylene and yields excellent results in automated tissue processing. Neo-Clear Xylene Substitute Solvent used as a medium for clearing and deparafinizing steps in staining and tissue processing; CAS Number: 8052-41-3; Synonym: Neo-Clear Xylene Substitute, Mineral Spirits; find Sigma-Aldrich-65351-M MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. Mr. Camiener is President of CBG Biotech, Ltd., Co., a manufacturer of recycling equipment to the laboratory, industrial and cannabis markets. Product documentation It is recommended that you allow all cookies. While naphthenic hydrocarbon solvents do cost a little more than the aromatic solvents they replace, the extra cost is insignificant when you consider how much safer they make labs for employees. Furthermore its low risk of igniting makes it safer to work with. It is less oily than other solvent substitutes and therefore dries quickly and does not leave a residue.. Clearene penetrates tissue rapidly without overhardening and is safe to use with all open and closed system tissue processors. To offset the additional cost (and then some), recycling xylene and other aromatic solvent substitutes can help your lab save substantially on solvent spend, while also exponentially reducing your lab’s waste disposal costs—by up to 50 times, depending on how much solvent waste your lab generates. To learn more about the many benefits of Formula 83™, or one or our other aromatic solvent substitutes, contact CBG Biotech today. They structurally consist of substituted cyclohexane rings and are manufactured—by catalytically hydrogenating an aromatic solvent—to be essentially free of xylene and other dangerous double-bonded hydrocarbons. Lab Tech Health & Well Being. Clearene is a selected blend of terpenes formulated specifically to replace xylene and toluene in histology and cytology laboratory procedures. Histosol is the original high flash point (114 degree F TCC) histological clearing agent. Clear (22) Light Yellow (2) Composition. ... Clear, Shandon Xylene Substitute (4 per Case) Qty Price: $356.12 / CS Available: Not In Stock. The majority of cell and tissue components have no natural color and are not visible. Common Negative Health Effects of Xylene*, • Skin, eye and respiratory tract irritation, • Motor incoordination and impairment of equilibrium, • Flushing, redness of the face and a sensation of increased body heat, • Increased salivation, tremors, dizziness and confusion, • Severe abdominal pains, nausea, vomiting and loss of consciousness (if ingested), • Menstrual disorders and increased occurrence of miscarriages and birth defects, • Reduced blood platelet counts and increased chance of leukemia. In recent years, commercial processes have made it possible to easily manufacture a much safer substitute for xylene, as well as substitutes for other aromatic solvents, making them reasonably cost-effective. References: 1. 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